Effect of Low–Level Helium-Neon Laser Irradiation on the Release of Interleukin 6 and Basic Fibroblast Growth Factor from Cultured Human Fibroblasts in High Glucose Medium

Purpose: Low level laser therapy is suggested as a new therapeutic method in diabetic wound healing. This survey aimed to evaluate the effects of low level laser on human fibroblasts cultured in high glucose cultures. Materials and Methods: The human skin fibroblasts were cultured under standard condition. The cells were cultured in high glucose culture medium (15mM/L) for a week and two weeks before laser irradiation. The irradiation was carried out with Helium-Neon (He-Ne) laser unit on the case groups. Control cells did not receive laser. The cells were irradiated with three doses of 0.5, 1 and 2 J/cm2 on three consecutive days. The releasing rates of Interleukin 6 and Fibroblast growth factor were evaluated by ELISA (enzyme-linked immunoabsorbent assay). Data were analyzed by student t test or Mann-Whitney-U test. Results: All three doses of 0.5 (p=0.049), 1 J/cm2 (p=0.027) had a stimulatory effect on releasing rate of IL-6 of the fibroblasts cultured in culture medium with high glucose concentration in comparison with hyper glycemic non irradiated cells. Low level laser therapy (LLLT) with energy density of 2 J/cm2 induced the release of bFGF of the fibroblasts cultured in culture medium with high glucose concentration for one and two weeks (p=0.047 and p=0.04 respectively). Conclusion: This study showed that LLLT has stimulatory effects on the release of Interleukin 6 and basic fibroblast growth factor from cultured human fibroblasts in high glucose medium in one week and two-week tests.